Malus pumila (fruit)

From AHPA Botanical Identity References Compendium
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=Introduction=
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{{DISPLAYTITLE:''Malus pumila'' (fruit) }} {{askbox|herb=''Malus pumila''}}
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=Nomenclature=
  
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=Macroscopic Entries=
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{{nomenclature | binomial=Malus pumila
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|authority=Mill.
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|family=Rosaceae
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|scn=apple
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|syn=''Malus domestica'' Borkh.; ''Pyrus pumila'' (Mill.) K. Koch
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|ayurvedic=
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|pinyin=
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|aka=cultivated apple
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|notes= }}
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=Botanical Voucher Specimen=
  
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=Microscopic Entries=
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{{Media      |cat=Voucher
  
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=HPTLC Entries=
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            | source=MOBOT, Tropicos.org
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            | mainimage=Malus_pumila_Tropicos_100108223_(S).jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100108223
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            | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100108223>
  
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=Other Points of Interest=
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            | }}
  
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=Organoleptic Characteristics=
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[[Category:Blank2]]
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[[Category:NoIntro]]
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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=High Performance Thin Layer Chromatographic Identification=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Apple (fruit) (''Malus pumila'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Malus pumila fruit - Alkemists Laboratories.jpg
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            | caption1=''Malus pumila'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=CHCl3: ethyl formate: HCOOH [6/1.5/1.5]
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            | prep=0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection= Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Malus pumila''-1 (entire)
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# 3 μL ''Malus pumila''-2 (fruit)
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# 3 μL ''Malus pumila''-3 (fruit)
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# 3 μL ''Malus pumila''-4 (fruit)
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# 3 μL ''Malus pumila''-4 (fruit)
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# 3 μL ''Malus pumila''-5 (fruit)
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# 3 μL ''Malus pumila''-6 (peel)
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# 3 μL Catechin ~0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Method Developed by Alkemists Laboratories
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 20:36, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Malus pumila.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Malus pumila Mill.   Rosaceae  
Syn. Malus domestica Borkh.; Pyrus pumila (Mill.) K. Koch  
Standardized common name (English): apple

Botanical Voucher Specimen

bottomright

Malus pumila Tropicos 100108223 (S).jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Malus pumila HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Apple (fruit) (Malus pumila)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Malus pumila-1 (entire)
  2. 3 μL Malus pumila-2 (fruit)
  3. 3 μL Malus pumila-3 (fruit)
  4. 3 μL Malus pumila-4 (fruit)
  5. 3 μL Malus pumila-4 (fruit)
  6. 3 μL Malus pumila-5 (fruit)
  7. 3 μL Malus pumila-6 (peel)
  8. 3 μL Catechin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: ethyl formate: HCOOH [6/1.5/1.5] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [2]

Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100108223
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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