Malus pumila (fruit)

Nomenclature

Malus pumila Mill.   Rosaceae  
Syn. Malus domestica Borkh.; Pyrus pumila (Mill.) K. Koch  
Standardized common name (English): apple

Botanical Voucher Specimen

Source: MOBOT, Tropicos.org

High Performance Thin Layer Chromatographic Identification

Malus pumila HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm Apple (fruit) (Malus pumila) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Malus pumila-1 (entire) 3 μL Malus pumila-2 (fruit) 3 μL Malus pumila-3 (fruit) 3 μL Malus pumila-4 (fruit) 3 μL Malus pumila-4 (fruit) 3 μL Malus pumila-5 (fruit) 3 μL Malus pumila-6 (peel) 3 μL Catechin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase CHCl3: ethyl formate: HCOOH [6/1.5/1.5]  Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Method Developed by Alkemists Laboratories Source: Elan M. Sudberg, Alkemist Laboratories