Malus pumila HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Apple (fruit) (Malus pumila)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Malus pumila-1 (entire)
2. 3 μL Malus pumila-2 (fruit)
3. 3 μL Malus pumila-3 (fruit)
4. 3 μL Malus pumila-4 (fruit)
5. 3 μL Malus pumila-4 (fruit)
6. 3 μL Malus pumila-5 (fruit)
7. 3 μL Malus pumila-6 (peel)
8. 3 μL Catechin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: ethyl formate: HCOOH [6/1.5/1.5] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories

Source: Elan M. Sudberg, Alkemist Laboratories ^[1]