Juglans nigra (hull)

Nomenclature

Juglans nigra L.   Juglandaceae  
Standardized common name (English): black walnut

Botanical Voucher Specimen

Source: MOBOT, Tropicos.org

High Performance Thin Layer Chromatographic Identification

Juglans nigra HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm Black Walnut (Hull) (Juglans nigra) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 5 μL Catechin~0.1% in CH3OH 5 μL Juglans nigra-1 (hull) 5 μL Juglans nigra-2 (hull) 5 μL Juglans nigra-3 (hull) 5 μL Juglans nigra-4 (hull) 5 μL Juglans nigra-4 (hull) 5 μL Juglans nigra-5 (hull) 5 μL Juglans nigra-6 (hull) Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: ethyl formate: formic acid [5/4/1]  Sample Preparation Method 0.3g+3mL 50% grain EtOH sonicate/heat @~50° C ~ 1/2 hr.  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Method Developed by Alkemists Laboratories Source: Elan M. Sudberg, Alkemist Laboratories