Handroanthus impetiginosus (bark)

Botanical Voucher Specimen

Source: Royal Botanic Gardens, Kew.	Source: Royal Botanic Gardens, Kew.

Microscopic Characteristics

Fragment of the bark showing dispersal of medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Bark fiber showing calcium oxalate prisms spaced by medullary rays observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Handroanthus impetiginosus HPTLC ID - 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm Pau D’Arco (bark) (Handroanthus impetiginosus) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Handroanthus impetiginosus-1 (bark) 3 μL Handroanthus impetiginosus-2 (bark) 3 μL Handroanthus impetiginosus-3 (bark) 3 μL Handroanthus impetiginosus-4 (bark) 3 μL Handroanthus impetiginosus-4 (bark) 3 μL Handroanthus impetiginosus-5 (bark) 3 μL Handroanthus impetiginosus-6 (bark) 1 μL Artemisinin ~0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase toluene: ethyl acetate: formic acid [7.5/2.5/0.5]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr.  Detection Method 10% Ethanolic H2SO4 ->115° C 15 min -> UV 365 nm  Reference see Plant Drug Analysis, Wagner, H., 1996 Source: Elan M. Sudberg, Alkemist Laboratories