Centella asiatica (aerial parts)

Nomenclature

Centella asiatica (L.) Urb.   Apiaceae  
Syn. Hydrocotyle asiatica L.  
Standardized common name (English): gotu kola  
Ayurvedic name(s): brahmi; mandukaparni  
Pinyin name(s): ji xue cao; ji xue cao (whole plant); luo-de-da (whole plant); ma ti cao (whole plant)

Botanical Voucher Specimen

Source: MOBOT, Tropicos.org	Source: MOBOT, Tropicos.org	Source: MOBOT, Tropicos.org

Source: Royal Botanic Gardens, Kew.	Source: Royal Botanic Gardens, Kew.

Macroscopic Characteristics

Source: PlantaPhile	Source: PlantaPhile

Microscopic Characteristics

Cellular structures identified in this botanical specimens are the rosettes of calcium oxalate found within the palisade cells below leaf epidermis and the epidermis of the leaf showing striated cuticle and numerous stomata. Source: Elan M. Sudberg, Alkemist Laboratories Rosettes of calcium oxalate found within the palisade cells below leaf epidermis observed at 400x with Acidified Chloral Hydrate Glycerol Solution Source: Elan M. Sudberg, Alkemist Laboratories Epidermis of the leaf showing striated cuticle and numerous stomata observed at 400x with Acidified Chloral Hydrate Glycerol Solution Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Centella asiatica HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm Gotu Kola (aerial parts) (Centella asiatica) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Asiaticoside ~0.1% in Methanol 3 μL Centella asiatica-1 (herb) 3 μL Centella asiatica-2 (aerial parts) 3 μL Centella asiatica-3 (aerial parts) 3 μL Centella asiatica-3 (aerial parts) 3 μL Centella asiatica-4 (herb) 3 μL Centella asiatica-5 (herb) 3 μL Asiaticoside ~0.1% in Methanol Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: glacial acetic acid: methanol: water [6/3.2/1.2/0.8]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see W.H.O. Monographs Vol. #1 1999 Source: Elan M. Sudberg, Alkemist Laboratories