Salvia officinalis (leaf)
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Nomenclature
Salvia officinalis L. Lamiaceae
Standardized common name (English): sage
Botanical Voucher Specimen
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Organoleptic Characteristics
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Macroscopic Characteristics
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Microscopic Characteristics
High Performance Thin Layer Chromatographic Identification
Sage (leaf) (Salvia officinalis) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference Sample(s) Reference: Dissolve 5 mg of thujon in 1 mL of methanol; Dissolve 25 µL of cineole in 10 mL of toluene. Stationary Phase Stationary phase, i.e. Silica gel 60, F254 Mobile Phase Dichloromethane Sample Preparation Method Sample: Mix 500 mg of powdered sample with 5 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. Derivatization reagent: Anisaldehyde reagent, Preparation: 170 mL of ice-cooled methanol add 20 mL of glacial acetic acid, 10 mL of sulfuric acid and 1 mL of anisaldehyde, Use: spray, heat at 100°C for 5 min Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test: Thujon: violet zone at Rf ~ 0.47; Cineole: violet zone at Rf ~ 0.27. Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a violet zone just below the solvent front. A weak yellow zone is seen at Rf ~ 0.50 (green arrow). There is a violet zone at Rf ~ 0.30 and slightly below reference cineole there is another violet zone. A yellow zone is seen at Rf ~ 0.20 and there is a blue violet zone just below it. Test for other species: No violet zone is seen at the position of thujon (Three-lobed sage leaf, blue arrow).
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Sage oil (leaf) (Salvia officinalis) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference Sample(s) Reference: Dissolve 1 mg of thujon in 1 mL of toluene. Dissolve 30 µL of cineole in 10 mL of toluene. Stationary Phase Stationary phase, i.e. Silica gel 60, F254 Mobile Phase Dichloromethane Sample Preparation Method Sample: Mix 100 µL of sample with 10 mL of toluene. Derivatization reagent: Anisaldehyde reagent, Preparation: To 170 mL of ice-cooled methanol add 20 mL of acetic acid, 10 mL of sulfuric acid and 1 mL of anisaldehyde, Use: spray, heat at 100°C for 5 min. Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes. System suitability test: Thujon: weak pink zone at Rf ~ 0.47; Cineole: violet zone at Rf ~ 0.27. Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows a grey zone at Rf ~ 0.54. At the position of reference thujon there is a diffuse pink zone (black arrow). Below it there is another pink zone. Just below the position of reference cineole a violet zone is seen. Below this zone there is an intense grey zone at Rf ~ 0.20. Test for other species: No zone is seen below the grey zone at Rf ~ 0.20 (green arrow, Clary sage oil). The chromatograms of Spanish sage oil and Clary sage oil lack the zone at the position of thujon.
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Supplementary Information
Sources
- ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100268968
- ↑ United States Dispensatory (1918)
- ↑ United States Dispensatory (1918)
- ↑ PlantaPhile http://plantaphile.com/
- ↑ PlantaPhile http://plantaphile.com/
- ↑ HPTLC Association http://www.hptlc-association.org/
- ↑ HPTLC Association http://www.hptlc-association.org/