Rehmannia glutinosa (root)

Nomenclature

Rehmannia glutinosa Libosch. ex Fisch. & Mey.   Scrophulariaceae  
Standardized common name (English): rehmannia  
Pinyin name(s): di huang; sheng di huang (unprocessed root tuber); shu di huang (cured root tuber)

Microscopic Characteristics

Parenchyma showing red pigmented material observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories	Vessels showing circular perforation observed at 400x with Acidified Chloral Hydrate Glycerol Solution. Source: Elan M. Sudberg, Alkemist Laboratories

High Performance Thin Layer Chromatographic Identification

Rehmannia glutinosa HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm Rehmannia (root) (Rehmannia glutinosa) Lane Assignments Lanes, from left to right (Track, Volume, Sample): 3 μL Rehmannia glutinosa-1 (root) 3 μL Rehmannia glutinosa-2 (root) 3 μL Rehmannia glutinosa-3 (root) 3 μL Rehmannia glutinosa-4 (root) 3 μL Rehmannia glutinosa-4 (root) 3 μL Rehmannia glutinosa-5 (root) 3 μL Rehmannia glutinosa-6 (root) 2 μL Catalpol ~ 0.1% in CH3OH Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.  Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates  Mobile Phase chloroform: methanol: water [7/3/0.5]  Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr  Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm  Reference see Japanese Official Monographs part III Source: Elan M. Sudberg, Alkemist Laboratories