Magnolia officinalis (bark)

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Latest revision as of 14:52, 26 May 2015

AHPA recognizes other valuable resources exist regarding the identity of Magnolia officinalis.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Magnolia officinalis Rehder & E.H. Wilson   Magnoliaceae  
Standardized common name (English): magnolia  
Pinyin name(s): hou po; hou po (bark of stem; twig; and root); hou po hua (flower bud)

Botanical Voucher Specimen

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Magnolia officinalis Kew barcode=K000681415 308529.jpg
Source: Royal Botanic Gardens, Kew.[1]

Organoleptic Characteristics

[The bark of Magnolia officinalis is] inodorous; taste somewhat astringent, pungent, and bitter.

The aromatic property, which resides in a volatile principle, is diminished by desiccation, and entirely lost when the bark is long kept. The bitterness, however, remains. The bark is destitute of astringency.
Source: United States Dispensatory (1918) [2]

Macroscopic Characteristics

The bark from young wood is quilled or curved, thin, externally orange-brown and glossy, or light gray, with scattered warts and somewhat fissured, internally whitish or pale brownish and smooth; fracture short, in the inner layer somewhat fibrous ...

The bark of old wood, deprived of the cork, is whitish or brownish, fibrous, and less pungent.

Source: United States Dispensatory (1918) [3]

Microscopic Characteristics

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Magnolia officinalis - Alkemist Laboratories.jpg
Cells containing yellow-brown oil observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[4]

Magnolia officinalis-2 - Alkemist Laboratories.jpg
Star shaped lignified sclereids observed at 400x with phloroglucinol + HCl.
Source: Elan M. Sudberg, Alkemist Laboratories[5]

Magnolia officinalis-3 - Alkemist Laboratories.jpg
Lignified fibers observed at 400x with phloroglucinol + HCl.
Source: Elan M. Sudberg, Alkemist Laboratories[6]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Magnolia officinalis HPTLC ID - 10% Ethanolic H2SO4 visible light

Magnolia (bark) (Magnolia officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Magnolol ~ 0.1% in Methanol
  2. 3 μL Magnolia officinalis-1
  3. 3 μL Magnolia officinalis-2
  4. 3 μL Magnolia officinalis-3
  5. 3 μL Magnolia officinalis-3
  6. 4 μL Magnolia officinalis-4
  7. 2 μL Magnolia officinalis-4
  8. 3 μL Honokiol ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase cyclohexane: ethyl acetate: HCOOH [6/4/0.2] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @~50° C ~ 1/2 hr 

Detection Method 10% Ethanolic H2SO4 -> 115° C 15 min -> visible light 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 1997


Source: Elan M. Sudberg, Alkemist Laboratories [7]

Supplementary Information

Sources

  1. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000681415
  2. ↑ United States Dispensatory (1918)
  3. ↑ United States Dispensatory (1918)
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  5. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  6. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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