Glycine max (bean)

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=Macroscopic Characteristics=
 
=Macroscopic Characteristics=
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{{Macroscopy | source=United States Dispensatory (1918)
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| description=[''Glycine max''] is an annual having hairy, trifoliate leaves, inconspicuous, violet-colored flowers, and forms broad legumes containing 2 to 5 seeds. The latter are compressed, spherical and vary from brownish-green to brownish-black.
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=Microscopic Characteristics=
 
=Microscopic Characteristics=
 
{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories

Latest revision as of 20:43, 24 March 2015

AHPA recognizes other valuable resources exist regarding the identity of Glycine max.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Glycine max Merr.   Fabaceae  
Standardized common name (English): soy bean

Botanical Voucher Specimen

bottomright

Glycine max Tropicos 100005223.jpg
Source: MOBOT, Tropicos.org[1]

Organoleptic Characteristics

Macroscopic Characteristics

[Glycine max] is an annual having hairy, trifoliate leaves, inconspicuous, violet-colored flowers, and forms broad legumes containing 2 to 5 seeds. The latter are compressed, spherical and vary from brownish-green to brownish-black.

Source: United States Dispensatory (1918) [2]

Microscopic Characteristics

bottomright bottomright

Soy Bean.jpg
Conical/dumbbell shaped (of various sizes) unicellular thick-walled fiber observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[3]

Soy Bean-1.jpg
Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[4]


High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Glycine max HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Soy (bean) (Glycine max)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Aescin ~0.1% in Methanol
  2. 3 μL Glycine max-1
  3. 3 μL Glycine max-2
  4. 3 μL Glycine max-3
  5. 3 μL Glycine max-3
  6. 3 μL Glycine max-4
  7. 3 μL Glycine max-5
  8. 3 μL Daidzein ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: Acetic acid: CH3OH: H2O [6/1.5/1.5/1] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories


Source: Elan M. Sudberg, Alkemist Laboratories [5]

Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100005223
  2. ↑ United States Dispensatory (1918)
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  4. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  5. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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