Cucurbita pepo (seed)
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=Introduction= | =Introduction= | ||
=Macroscopic Entries= | =Macroscopic Entries= | ||
| + | {{Macroscopy | source=United States Dispensatory (1918) | ||
| + | | description=''Cucurbita Pepo'', or common pumpkin, is a plant almost too well | ||
| + | known to need description. | ||
| + | The seeds are broadly elliptical or ovate, from 15 to 23 | ||
| + | mm. in length and from 2 to 3 mm. in thickness; externally yellowish-white, | ||
| + | very smooth, occasionally with thin, transparent fragments of | ||
| + | adhering pulp and with a shallow groove parallel to and within 1 mm. | ||
| + | of the margin; fracture short, seed-coat consisting of a white coriaceous | ||
| + | outer layer and a membranous inner layer occasionally of a dark green | ||
| + | color; embryo whitish, straight, with a small conical hypocotyl and two | ||
| + | plano-convex cotyledons; slightly odorous when contused; taste bland | ||
| + | and oily. Under the microscope, sections of Pepo show an outer | ||
| + | epidermal layer consisting of palisade-like cells, the radial walls | ||
| + | attaining a length of 1 mm., the outer walls being usually torn off so | ||
| + | that it appears as though the seeds were covered with very long hairs; a | ||
| + | sub-epidermal layer consisting- of from 5 to 12 rows of cells with slightly | ||
| + | thickened, lignified and porous walls; a layer of strongly lignified stone | ||
| + | cells, elliptical in outline and about 0.075 mm. in length; a single layer | ||
| + | of small cells resembling those of the sub-epidermal layer; several rows | ||
| + | of parenchyma cells with characteristic reticulate markings and | ||
| + | separated from each other by large intercellular spaces; several layers of | ||
| + | parenchyma cells, the inner layer being more or less collapsed and | ||
| + | bounded on the inside by a single epidermal layer, the cells having | ||
| + | rather thick walls; the perisperm cells are usually more or less collapsed | ||
| + | and the endosperm consists of a single layer of cells filled with small | ||
| + | aleurone grains; the cotyledons consist of thin-walled, isodiametric, | ||
| + | elongated or palisade-like cells containing a fixed oil and numerous | ||
| + | small aleurone grains.}} | ||
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=Microscopic Entries= | =Microscopic Entries= | ||
=HPTLC Entries= | =HPTLC Entries= | ||
Revision as of 21:08, 31 December 2013
Contents |
Introduction
Macroscopic Entries
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Microscopic Entries
HPTLC Entries
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Pumpkin (seed) (Cucurbita pepo) Lane Assignments Lanes, from left to right (Track, Volume, Sample):
Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates Mobile Phase chloroform: ethanol [9.5/0.5] Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> visible light Reference see British Herbal Pharmacopoeia, 1996
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Other Points of Interest
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