Ganoderma lucidum HPTLC ID - 10% Methanolic Sulfuric Acid - UV 365 nm

Reishi (fruiting body) (Ganoderma lucidum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Ursolic Acid ~ 0.1% Methanol
2. 3 μL Ganoderma lucidum-1 (fruiting body)
3. 3 μL Ganoderma lucidum-2 (fruiting body)
4. 3 μL Ganoderma lucidum-3 (fruiting body)
5. 3 μL Ganoderma lucidum-3 (fruiting body)
6. 3 μL Ganoderma lucidum-4 (fruiting body)
7. 3 μL Ganoderma lucidum-5 (fruiting body)
8. 3 μL Ganoderma lucidum-6 (fruiting body)

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase Dichloromethane : Methanol [9/1] 

Sample Preparation Method 0.3g+3mL 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method 10% Methanolic H2SO4 -> UV 365 nm 

Reference see American Herbal Pharmacopoeia & Therapeutic Compendium

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]