Carthamus tinctorius L.
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden^[1]

Carthamus tinctorius L.
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden^[2]

Carthamus tinctorius L.
Source: Images courtesy of the C.V. Starr Virtual Herbarium of the New York Botanical Garden^[3]

Fragment of the corolla showing brown laticiferous vessels observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[6]

Three pored pollen grain shown exuding contents observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[7]

Carthamus tinctorius HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Safflower (flower) (Carthamus tinctorius)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
2. 3 μL Carthamus tinctorius-1 (flower)
3. 3 μL Carthamus tinctorius-2 (flower)
4. 3 μL Carthamus tinctorius-3 (flower)
5. 3 μL Carthamus tinctorius-3 (flower)
6. 3 μL Carthamus tinctorius-4 (flower)
7. 3 μL Carthamus tinctorius-5 (flower)
8. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: AcCOOH: HCOOH: H2O [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Adapted from Plant Drug Analysis, Wagner, H., 1996

Source: Elan M. Sudberg, Alkemist Laboratories ^[8]