Parenchyma cell containing crystal sand with small prisms observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[1]

Cyathula officinalis HPTLC ID - 10% Methanolic H2SO4 365 nm

Cyathula (root) (Cyathula officinalis)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Sitosterol ~0.1% in CH3OH
2. 2 μL Cyathula officinalis
3. 4 μL Cyathula officinalis
4. 3 μL Cyathula officinalis
5. 3 μL Cyathula officinalis
6. 4 μL Cyathula officinalis
7. 2 μL Cyathula officinalis
8. 3 μL Escin ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase CHCl3: Acetic acid: CH3OH: H2O [6/3.2/1.2/0.8] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate + heat @ 50° C ~ 1/2 hr 

Detection Method 10% Methanolic H2SO4 -> 115° C 15 min -> UV 365 nm 

Reference see Pharmacopoeia of The Peoples Republic of China, Volume 1, 2005

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]