Carthamus tinctorius (flower)

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Revision as of 21:13, 15 March 2014


Contents

Nomenclature

Carthamus tinctorius L.   Asteraceae  
Standardized common name (English): safflower  
Ayurvedic name(s): kusumbha  
Pinyin name(s): hong hua (flower)

Macroscopic Characteristics

Carthamus tinctorius L. Safflower.—

The African, false, American, or dyers' saffron is an annual composite, with a smooth, erect stem, somewhat branched at top, and a foot or two in height. ... The florets are in mass of a red color, diversified by the yellow of the styles contained within the floret. It has a peculiar, slightly aromatic odor, and a scarcely perceptible bitterness.

It contains a fixed oil; also two coloring substances—one red, insoluble in alkaline liquids, and called carthamin or carthamic acid by Dobereiner, who found it to possess weak acid properties; the other yellow, and soluble in water.

Source: United States Dispensatory (1918) [1]

Microscopic Characteristics

Fragment of the corolla showing brown laticiferous vessels observed at 400x with Acidified Chloral Hydrate Glycerol Solution.cellular structures identified in this botanical specimen are fragment of the corolla showing brown laticiferous vessels and three pored pollen grain shown exuding contents when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [2]
AP-LOGO-Laboratories Crop - Copy.jpg
Safflower Alkemist Laboratories.jpg



High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Carthamus tinctorius HPTLC ID - Natural Product Reagent + PEG UV 365 nm

Safflower (flower) (Carthamus tinctorius)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol
  2. 3 μL Carthamus tinctorius-1 (flower)
  3. 3 μL Carthamus tinctorius-2 (flower)
  4. 3 μL Carthamus tinctorius-3 (flower)
  5. 3 μL Carthamus tinctorius-3 (flower)
  6. 3 μL Carthamus tinctorius-4 (flower)
  7. 3 μL Carthamus tinctorius-5 (flower)
  8. 1 μL Rutin, Caffeic Acid, Hyperoside, Chlorogenic Acid ~ 0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: AcCOOH: HCOOH: H2O [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Adapted from Plant Drug Analysis, Wagner, H., 1996


Source: Elan M. Sudberg, Alkemist Laboratories [3]


Supplementary Information


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