Rehmannia glutinosa (root)

From AHPA Botanical Identity References Compendium
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=Introduction=
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{{DISPLAYTITLE:''Rehmannia glutinosa'' (root) }} {{askbox|herb=''Rehmannia glutinosa''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{nomenclature | binomial=Rehmannia glutinosa
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=HPTLC Entries=
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|authority=Libosch. ex Fisch. & Mey.
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=Other Points of Interest=
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|family=Scrophulariaceae
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[[Category:blank]]
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|scn=rehmannia
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|syn=
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|ayurvedic=
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|pinyin=di huang; sheng di huang (unprocessed root tuber); shu di huang (cured root tuber)
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|aka=
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|notes= }}
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=Botanical Voucher Specimen=
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Rehmannia glutinosa - Alkemist Laboratories.jpg
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            | caption1=Parenchyma showing red pigmented material  observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
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            | image2=Rehmannia glutinosa-1 - Alkemist Laboratories.jpg
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            | caption2=Vessels showing circular perforation observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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            | reference=Japanese Official Monographs part III
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            | }}
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=High Performance Thin Layer Chromatographic Identification=
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{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
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            | description=Rehmannia (root) (''Rehmannia glutinosa'')
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            | companyimage=AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL=http://www.alkemist.com
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            | mainimage=Rehmannia glutinosa root - Alkemists Laboratories.jpg
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            | caption1=''Rehmannia glutinosa'' HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | stationaryphase=Silica gel 60, F254, 10 x 10 cm HPTLC plates
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            | mobilephase=chloroform: methanol: water [7/3/0.5]
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            | prep=0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr
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            | detection=Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm
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            | lanes= Lanes, from left to right (Track, Volume, Sample):
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# 3 μL ''Rehmannia glutinosa''-1 (root)
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# 3 μL ''Rehmannia glutinosa''-2 (root)
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# 3 μL ''Rehmannia glutinosa''-3 (root)
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# 3 μL ''Rehmannia glutinosa''-4 (root)
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# 3 μL ''Rehmannia glutinosa''-4 (root)
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# 3 μL ''Rehmannia glutinosa''-5 (root)
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# 3 μL ''Rehmannia glutinosa''-6 (root)
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# 2 μL Catalpol ~ 0.1% in CH3OH
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
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            | reference=Japanese Official Monographs part III
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            | }}
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=Supplementary Information=
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=Sources=
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<references />

Latest revision as of 21:03, 2 June 2014

AHPA recognizes other valuable resources exist regarding the identity of Rehmannia glutinosa.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Rehmannia glutinosa Libosch. ex Fisch. & Mey.   Scrophulariaceae  
Standardized common name (English): rehmannia  
Pinyin name(s): di huang; sheng di huang (unprocessed root tuber); shu di huang (cured root tuber)

Botanical Voucher Specimen

Organoleptic Characteristics

Macroscopic Characteristics

Microscopic Characteristics

bottomright bottomright

Rehmannia glutinosa - Alkemist Laboratories.jpg
Parenchyma showing red pigmented material observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[1]

Rehmannia glutinosa-1 - Alkemist Laboratories.jpg
Vessels showing circular perforation observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[2]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Rehmannia glutinosa HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Rehmannia (root) (Rehmannia glutinosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 3 μL Rehmannia glutinosa-1 (root)
  2. 3 μL Rehmannia glutinosa-2 (root)
  3. 3 μL Rehmannia glutinosa-3 (root)
  4. 3 μL Rehmannia glutinosa-4 (root)
  5. 3 μL Rehmannia glutinosa-4 (root)
  6. 3 μL Rehmannia glutinosa-5 (root)
  7. 3 μL Rehmannia glutinosa-6 (root)
  8. 2 μL Catalpol ~ 0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [7/3/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Japanese Official Monographs part III


Source: Elan M. Sudberg, Alkemist Laboratories [3]

Supplementary Information

Sources

  1. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  2. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  3. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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