Malva sylvestris (flower)

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=Introduction=
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{{DISPLAYTITLE:''Malva sylvestris'' (flower)}} {{askbox|herb=''Malva sylvestris''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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=Botanical Voucher Specimen=
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{{Media3      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Malva_sylvestris_Tropicos_100000537.jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100000537
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            | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100000537>
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| companyimage2=Kewlogo.gif
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| companyURL2=http://specimens.kew.org/herbarium/K000914119
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| image2=Malva sylvestris Kew barcode=K000914119 479685.jpg
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| source2=Royal Botanic Gardens, Kew.
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| companyimage3=Kewlogo.gif
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| companyURL3=http://specimens.kew.org/herbarium/K000659331
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| image3=Malva sylvestris L. var. violascens Kew barcode=K000659331 284874.jpg
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|caption3=''Malva sylvestris'' L. var. ''violascens''
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| source3=Royal Botanic Gardens, Kew.
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            | }}
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=Organoleptic Characteristics=
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{| border=1
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|
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{{Organolepsy | source=Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
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            | description=Slight odor; sweetish, mucilaginous taste.}}
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|}
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=Macroscopic Characteristics=
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{| border=1
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|
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{{Macroscopy | source=Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
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| description=The flowers are rose-red, purple, or whitish, but when dried bluish, green with ammonia, red with acids.
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}}
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|}
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=Microscopic Characteristics=
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{| border=1
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|
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{{Microscopy | source= Elan M. Sudberg, Alkemist Laboratories
 
       | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
       | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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       | companyURL=http://www.Alkemist.com
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       | companyURL= http://www.alkemist.com
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      | mainimage=Blue_Mallow_Flower_Alkemist_Laboratories.jpg
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| description= Cellular structures identified in this botanical specimen are spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine and the thick walled stellate trichomes when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | caption1=Spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | description= High Mallow (flower) (''Malva sylvestris'')
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      | image2=Blue_Mallow_Flower-1_Alkemist_Laboratories.jpg
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      | caption2=Thick walled stellate trichomes observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | characteristics=cellular structures identified in this botanical specimen are spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine and the thick walled stellate trichomes when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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             | reference=British Pharmacopoeia, 2003
 
             | reference=British Pharmacopoeia, 2003
 
       | }}
 
       | }}
  
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=HPTLC Entries=
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{{Media2 |cat=Microscopy |companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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=Other Points of Interest=
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            | companyURL= http://www.alkemist.com
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|companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2= http://www.alkemist.com
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|mainimage= Blue_Mallow_Flower_Alkemist_Laboratories.jpg
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          |caption1=Spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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          |source= Elan M. Sudberg, Alkemist Laboratories
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|image2= Blue_Mallow_Flower-1_Alkemist_Laboratories.jpg
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          |caption2=Thick walled stellate trichomes observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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          |source2= Elan M. Sudberg, Alkemist Laboratories }}
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|}
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=High Mallow (flower) (''Malva sylvestris'')
 
             | description=High Mallow (flower) (''Malva sylvestris'')
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             | }}
 
             | }}
  
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[[Category:blank]]
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{{HPTLC | source=HPTLC Association
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            | companyimage=HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
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            | companyURL=http://www.hptlc-association.org/
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            | mainimage=Malva sylvestris-hptclc-association.png
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            | caption1=Mallow (flower) HPTLC ID - White RT
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            | description=Mallow (flower) (''Malva sylvestris'')
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            |
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            | stationaryphase=Stationary phase, i.e. Silica gel 60, F254
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            | mobilephase=1-Butanol, formic acid, water 65:16:19 (v/v/v)
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            | prep=Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions.
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            | detection=Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33%
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            | referencesamples=Reference: Dissolve 2 mg of pelargonin in 5 mL of methanol. Dissolve 2 mg of delphinidin in 5 mL of methanol; Optional: dissolve 2 mg of malvidin in 5 mL of methanol.
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            |
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            | lanes=Lanes, from left to right (Track, Volume, Sample):
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# 2 µL Bilberry fruit (Ph.Eur. extraction)
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# 3 µL Bilberry fruit (Ph.Eur. extraction)
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# 4 µL Bilberry fruit (Ph.Eur. extraction)
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# 2 µL Bilberry fruit
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# 3 µL Bilberry fruit
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# 4 µL Bilberry fruit
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# 2 µL Pelargonin
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# 2 µL Malvidin
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# 2 µL Delphinidin
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# 2 µL Mallow flower
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# '''4 µL Mallow flower'''
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# 6 µL Mallow flower
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# 2 µL Roselle flower
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# 4 µL Roselle flower
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# 6 µL Roselle flower
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            | notes=''Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.''
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System suitability test: Pelargonin: red to orange zone at Rf ~ 0.89; Delphinidin: violet zone at Rf ~ 0.80
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Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows one intensive violet zone at Rf ~ 0.35.
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Test for other species: No intense violet-blue zones are seen between Rf ~ 0.46 and 0.55 (Bilberry fruit) and no violet zone is present at Rf ~ 0.44 (Roselle flower).
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            | }}
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=Supplementary Information=
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=Sources=
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<references />
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[[Category:Botanical]]

Latest revision as of 15:44, 7 July 2015

AHPA recognizes other valuable resources exist regarding the identity of Malva sylvestris.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Botanical Voucher Specimen

bottomright bottomright bottomright

Malva sylvestris Tropicos 100000537.jpg
Source: MOBOT, Tropicos.org[1]

Malva sylvestris Kew barcode=K000914119 479685.jpg
Source: Royal Botanic Gardens, Kew.[2]

Malva sylvestris L. var. violascens Kew barcode=K000659331 284874.jpg
Malva sylvestris L. var. violascens
Source: Royal Botanic Gardens, Kew.[3]

Organoleptic Characteristics

Slight odor; sweetish, mucilaginous taste.

Source: Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed. [4]

Macroscopic Characteristics

The flowers are rose-red, purple, or whitish, but when dried bluish, green with ammonia, red with acids.

Source: Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed. [5]

Microscopic Characteristics

Cellular structures identified in this botanical specimen are spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine and the thick walled stellate trichomes when observed at 400x with Acidified Chloral Hydrate Glycerol Solution.

Source: Elan M. Sudberg, Alkemist Laboratories [6]
AP-LOGO-Laboratories Crop - Copy.jpg


bottomright bottomright

Blue Mallow Flower Alkemist Laboratories.jpg
Spherical pollen grains, about 150 μm in diameter, with a roughly spiny exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[7]

Blue Mallow Flower-1 Alkemist Laboratories.jpg
Thick walled stellate trichomes observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[8]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Malva sylvestris HPTLC ID - Natural Product Reagent + PEG UV 365 nm

High Mallow (flower) (Malva sylvestris)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH
  2. μL Malva sylvestris-1 (flower)
  3. μL Malva sylvestris-1 (flower)
  4. μL Malva sylvestris-2 (flower)
  5. μL Malva sylvestris-2 (flower)
  6. μL Malva sylvestris-3 (flower)
  7. μL Malva sylvestris-4 Vouchered Specimen (flower)
  8. μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: HCOOH: AcCOOH: H2O [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Natural Product Reagent + PEG -> UV 365 nm 

Reference see Adapted from British Pharmacopoeia, 2003


Source: Elan M. Sudberg, Alkemist Laboratories [9]


HPTLC-assoc-Logo-farbig-Text-schwarz-300x47.png
(thumbnail)
Mallow (flower) HPTLC ID - White RT

Mallow (flower) (Malva sylvestris)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 2 µL Bilberry fruit (Ph.Eur. extraction)
  2. 3 µL Bilberry fruit (Ph.Eur. extraction)
  3. 4 µL Bilberry fruit (Ph.Eur. extraction)
  4. 2 µL Bilberry fruit
  5. 3 µL Bilberry fruit
  6. 4 µL Bilberry fruit
  7. 2 µL Pelargonin
  8. 2 µL Malvidin
  9. 2 µL Delphinidin
  10. 2 µL Mallow flower
  11. 4 µL Mallow flower
  12. 6 µL Mallow flower
  13. 2 µL Roselle flower
  14. 4 µL Roselle flower
  15. 6 µL Roselle flower 

Reference Sample(s) Reference: Dissolve 2 mg of pelargonin in 5 mL of methanol. Dissolve 2 mg of delphinidin in 5 mL of methanol; Optional: dissolve 2 mg of malvidin in 5 mL of methanol. 

Stationary Phase Stationary phase, i.e. Silica gel 60, F254 

Mobile Phase 1-Butanol, formic acid, water 65:16:19 (v/v/v) 

Sample Preparation Method Sample: Mix 1 g of powdered sample with 10 mL of methanol and sonicate for 10 minutes, then centrifuge or filter the solutions and use the supernatants / filtrates as test solutions. 

Detection Method Saturated chamber; developing distance 70 mm from lower edge; relative humidity 33% 

Other Notes Images presented in this entry are examples and are not intended to be used as basis for setting specifications for quality control purposes.

System suitability test: Pelargonin: red to orange zone at Rf ~ 0.89; Delphinidin: violet zone at Rf ~ 0.80

Identification: Compare result with reference images. The fingerprint of the test solution is similar to that of the corresponding botanical reference sample. Additional weak zones may be present. The chromatogram of the test solution shows one intensive violet zone at Rf ~ 0.35.

Test for other species: No intense violet-blue zones are seen between Rf ~ 0.46 and 0.55 (Bilberry fruit) and no violet zone is present at Rf ~ 0.44 (Roselle flower).


Source: HPTLC Association [10]

Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100000537
  2. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000914119
  3. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000659331
  4. ↑ Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
  5. ↑ Culbreth, D. (1917) A Manual of Materia Media and Pharmacology, 6th ed.
  6. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  8. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  9. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  10. ↑ HPTLC Association http://www.hptlc-association.org/
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