Medicago sativa (leaf)

From AHPA Botanical Identity References Compendium
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=Introduction=
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{{DISPLAYTITLE:''Medicago sativa'' (leaf) }} {{askbox|herb=''Medicago sativa''}}
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=Macroscopic Entries=
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=Nomenclature=
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=Microscopic Entries=
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{{nomenclature | binomial=Medicago sativa
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{{Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
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|authority=L.
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|family=Fabaceae
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|scn=alfalfa
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|syn=
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|ayurvedic=
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|pinyin=
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|aka=lucerne
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|notes=}}
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=Botanical Voucher Specimen=
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{{Media3      |cat=Voucher
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            | source=MOBOT, Tropicos.org
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            | mainimage=Medicago_sativa_Tropicos_100136132_(S).jpg
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            | companyimage=TropicosLogo.gif
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            | companyURL=http://www.tropicos.org/Image/100136132
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            | reference=Tropicos.org. Missouri Botanical Garden. 26 Mar 2014 <http://www.tropicos.org/Image/100136132>
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| companyimage2=Kewlogo.gif
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| companyURL2=http://specimens.kew.org/herbarium/K000226899
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| image2=Medicago sativa Kew barcode=K000226899 16978.jpg
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| source2=Royal Botanic Gardens, Kew.
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| companyimage3=Kewlogo.gif
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| companyURL3=http://specimens.kew.org/herbarium/K001122610
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| image3=Medicago sativa Kew barcode=K001122610 708349.jpg
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| source3=Royal Botanic Gardens, Kew.
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            | }}
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=Organoleptic Characteristics=
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=Macroscopic Characteristics=
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{{Media2 |cat=Macroscopy
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| source=PlantaPhile
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            | mainimage=PlantaPhile - 116.jpg
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            | companyimage=PlantaPhile logo.jpg
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            | companyURL=http://plantaphile.com/
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            |
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| source2=PlantaPhile
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            | image2=PlantaPhile_-_3044.jpg
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            | companyimage2=PlantaPhile logo.jpg
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            | companyURL2=http://plantaphile.com/
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            | }}
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=Microscopic Characteristics=
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{{Media2 |cat=Microscopy | source=Elan M. Sudberg, Alkemist Laboratories
 
       | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
 
       | companyimage= AP-LOGO-Laboratories Crop - Copy.jpg
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       | companyURL=http://www.Alkemist.com
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       | companyURL=http://www.alkemist.com
 
       | mainimage=Alfalfa Alkemist Laboratories.jpg
 
       | mainimage=Alfalfa Alkemist Laboratories.jpg
 
       | caption1=Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
       | caption1=Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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      | description= Alfalfa (leaf) (''Medicago sativa'')
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| source2=Elan M. Sudberg, Alkemist Laboratories
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            | companyimage2= AP-LOGO-Laboratories Crop - Copy.jpg
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            | companyURL2=http://www.alkemist.com
 
       | image2=Alfalfa-1 Alkemist Laboratories.jpg
 
       | image2=Alfalfa-1 Alkemist Laboratories.jpg
 
       | caption2=Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
 
       | caption2=Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
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       | }}
 
       | }}
  
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=HPTLC Entries=
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=High Performance Thin Layer Chromatographic Identification=
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
{{HPTLC | source=Elan M. Sudberg, Alkemist Laboratories
 
             | description=Alfalfa (leaf) (''Medicago sativa'' L.)
 
             | description=Alfalfa (leaf) (''Medicago sativa'' L.)
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# 3 μL Canavanine ~0.1% in CH3OH
 
# 3 μL Canavanine ~0.1% in CH3OH
  
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Pharmaceuticals, Costa Mesa, CA.
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Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA.
 
             | reference=Phytochemical Methods, Harborne, J.B., 1998, p. 198
 
             | reference=Phytochemical Methods, Harborne, J.B., 1998, p. 198
 
             | }}
 
             | }}
  
  
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=Other Points of Interest=
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=Supplementary Information=
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[[Category:blank]]
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=Sources=
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<references />

Latest revision as of 15:14, 26 May 2015

AHPA recognizes other valuable resources exist regarding the identity of Medicago sativa.

To submit a suggestion or contribution, please contact Merle Zimmermann.

Contents

Nomenclature

Medicago sativa L.   Fabaceae  
Standardized common name (English): alfalfa

Botanical Voucher Specimen

bottomright bottomright bottomright

Medicago sativa Tropicos 100136132 (S).jpg
Source: MOBOT, Tropicos.org[1]

Medicago sativa Kew barcode=K000226899 16978.jpg
Source: Royal Botanic Gardens, Kew.[2]

Medicago sativa Kew barcode=K001122610 708349.jpg
Source: Royal Botanic Gardens, Kew.[3]


Organoleptic Characteristics

Macroscopic Characteristics

bottomright bottomright

PlantaPhile - 116.jpg
Source: PlantaPhile[4]

PlantaPhile - 3044.jpg
Source: PlantaPhile[5]

Microscopic Characteristics

bottomright bottomright

Alfalfa Alkemist Laboratories.jpg
Long unicellular trichome showing warted exine observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[6]

Alfalfa-1 Alkemist Laboratories.jpg
Prisms of calcium oxalate observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories[7]

High Performance Thin Layer Chromatographic Identification

AP-LOGO-Laboratories Crop - Copy.jpg
(thumbnail)
Medicago sativa L. HPTLC ID - Ninhydrin Reagent, Visible Light

Alfalfa (leaf) (Medicago sativa L.)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

  1. 1 μL Arginine ~0.1% in CH3OH
  2. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  3. 3 μL ‘‘Medicago sativa’’ L. (herb)
  4. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  5. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  6. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  7. 3 μL ‘‘Medicago sativa’’ L. (leaf)
  8. 3 μL Canavanine ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase n-butanol: pyridine: AcCOOH: H2O [5/1.25/1.25/2] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicated + heated @ 50° C ~ 1 hr. 

Detection Method Ninhydrin Reagent -> visible light 

Reference see Phytochemical Methods, Harborne, J.B., 1998, p. 198


Source: Elan M. Sudberg, Alkemist Laboratories [8]


Supplementary Information

Sources

  1. ↑ MOBOT, Tropicos.org http://www.tropicos.org/Image/100136132
  2. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K000226899
  3. ↑ Royal Botanic Gardens, Kew. http://specimens.kew.org/herbarium/K001122610
  4. ↑ PlantaPhile http://plantaphile.com/
  5. ↑ PlantaPhile http://plantaphile.com/
  6. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  7. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
  8. ↑ Elan M. Sudberg, Alkemist Laboratories http://www.alkemist.com
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