Parenchyma showing red pigmented material observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[1]

Vessels showing circular perforation observed at 400x with Acidified Chloral Hydrate Glycerol Solution.
Source: Elan M. Sudberg, Alkemist Laboratories^[2]

Rehmannia glutinosa HPTLC ID - Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm

Rehmannia (root) (Rehmannia glutinosa)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Rehmannia glutinosa-1 (root)
2. 3 μL Rehmannia glutinosa-2 (root)
3. 3 μL Rehmannia glutinosa-3 (root)
4. 3 μL Rehmannia glutinosa-4 (root)
5. 3 μL Rehmannia glutinosa-4 (root)
6. 3 μL Rehmannia glutinosa-5 (root)
7. 3 μL Rehmannia glutinosa-6 (root)
8. 2 μL Catalpol ~ 0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase chloroform: methanol: water [7/3/0.5] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Japanese Official Monographs part III

Source: Elan M. Sudberg, Alkemist Laboratories ^[3]