Ulmus rubra HPTLC ID - Natural Product Reagent + PEG-> UV 365 nm

Slippery Elm (bark) (Ulmus rubra)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Ulmus rubra-1 (bark)
2. 3 μL Ulmus rubra-2 (bark)
3. 3 μL Ulmus rubra-3 (bark)
4. 3 μL Ulmus rubra-4 (bark)
5. 3 μL Ulmus rubra-4 (bark)
6. 3 μL Ulmus rubra-5 (bark)
7. 3 μL Ulmus rubra-6 (bark)
8. 1 μL Rutin, Caffeic acid, Hyperoside, Chlorogenic Acid ~0.1% in CH3OH

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: glacial acetic acid: formic acid: water [10/1.1/1.1/2.4] 

Sample Preparation Method 0.3 g + 3 ml CH3OH sonicate/heat @ 50° C ~ 1 hr. 

Detection Method Natural Product Reagent + PEG-> UV 365 nm 

Reference see British Herbal Pharmacopoeia, 1996

Source: Elan M. Sudberg, Alkemist Laboratories ^[1]