Glycine max HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Soy (bean) (Glycine max)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 3 μL Aescin ~0.1% in Methanol
2. 3 μL Glycine max-1
3. 3 μL Glycine max-2
4. 3 μL Glycine max-3
5. 3 μL Glycine max-3
6. 3 μL Glycine max-4
7. 3 μL Glycine max-5
8. 3 μL Daidzein ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase ethyl acetate: Acetic acid: CH3OH: H2O [6/1.5/1.5/1] 

Sample Preparation Method 0.3 g + 3 ml 70% grain EtOH sonicate/heat @ 50° C ~ 1/2 hr 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Method Developed by Alkemists Laboratories

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]