Coriandrum sativum HPTLC ID - Vanillin Sulfuric Acid Reagent UV 365 nm

Coriander (seed) (Coriandrum sativum)

Lane Assignments Lanes, from left to right (Track, Volume, Sample):

1. 1 μL Linalool ~0.1% in Methanol
2. 2 μL Coriandrum sativum-1 (seed)
3. 2 μL Coriandrum sativum-2 (seed)
4. 2 μL Coriandrum sativum-3 (seed)
5. 2 μL Coriandrum sativum-3 (seed)
6. 2 μL Coriandrum sativum-4 (seed)
7. 2 μL Coriandrum sativum-5 (seed)
8. 1 μL Linalool ~0.1% in Methanol

Reference materials used here have been authenticated by macroscopic, microscopic &/or TLC studies according to the reference source cited below held at Alkemists Laboratories, Costa Mesa, CA. 

Stationary Phase Silica gel 60, F254, 10 x 10 cm HPTLC plates 

Mobile Phase toluene: ethyl acetate [9.5/0.5] 

Sample Preparation Method 0.5g+5ml dichloromethane, sncte/cntrfge/dcnt, evap dry (N2), qs 1.0 ml Toluene 

Detection Method Vanillin/H2SO4 Reagent -> 110° C 5 min -> UV 365 nm 

Reference see Herbal Drugs and Phytopharmaceuticals,Max Wichtl, 3rd ed., 2004

Source: Elan M. Sudberg, Alkemist Laboratories ^[2]